FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4538853
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAACG3159.678843E-973.15001340-345
GTCGATA2651.13210364E-465.13604280-289
AGTAGCT88400.058.607795
CGAGTAG23900.057.5067063
GAGTAGC59300.055.423834
GTAGCTG93900.055.061546
CCGAGTA25450.054.7214052
TAGCTGG101450.051.9631087
GCTGGGA181400.051.7370579
AAGTGCT98350.046.177225
CCCGAGT31800.045.731921
TACGATA3857.293067E-444.66674330-339
TTGGGAG124700.043.6162156
AGTGCTG103150.043.178316
ATCCGTA3700.00507121542.00574340-345
GGAGGCT121650.041.8132559
CCAAAGT122050.041.2347532
CCCAAAG139050.041.1891
ACGACGG4702.0217652E-440.730953320-329
AAAGTGC111650.040.46244
Produced by FastQC (version 0.11.5)