FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4177002
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG13250.042.921512
CGAGTAG11550.042.7275123
TACTCGG13800.040.1886333
AAAGTGC69250.039.568454
GAGTAGC29750.039.3380364
CCCAAAG84400.039.172241
GCTGGGA114550.039.1415449
CCGAGTA13550.038.502522
AGTAGCT50450.038.195995
TAGCTGG53250.037.6880237
AAGTGCT72950.037.625535
CCAAAGT80750.036.7857862
TTGGGAG80650.036.5974126
CAAAGTG76900.035.99893
GTAGCTG54300.035.4878046
CCCGAGT15650.035.1590421
GGAGGCT86550.035.0249379
AGTGCTG80150.034.304216
GTGCTGG82000.033.3010067
TCGGGAG17900.031.7707486
Produced by FastQC (version 0.11.5)