FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4208876
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG14650.039.1791273
GAGTAGC33950.037.1387754
CCGAGTA16000.036.7651372
AGTAGCT53600.036.0752685
GCTGGGA113300.034.8410729
CTACTCG11300.034.1492842
TACTCGG11500.033.9557083
AAAGTGC63550.033.906464
GTAGCTG56450.033.466836
CCCAAAG81300.033.3910141
CCCGAGT19350.033.368541
AAGTGCT66850.032.654965
CCAAAGT74600.032.424292
TAGCTGG60500.032.390897
GCTACTC27800.032.38071
GGAGGCT81200.031.3100229
CAAAGTG75600.030.7423933
TTGGGAG78950.029.6435156
AGTGCTG72000.029.1108466
GTGCTGG75000.028.9482867
Produced by FastQC (version 0.11.5)