FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4208876
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG13400.045.597182
TACTCGG14100.044.666833
CGAGTAG13350.044.007653
CCGAGTA14300.042.727432
GAGTAGC32900.038.7144244
CCCAAAG82300.038.280331
GCTACTC33300.037.84351
AGTAGCT54250.037.6863255
GCTGGGA118950.037.4965139
CCCGAGT17750.036.028091
GTAGCTG57200.035.9892166
CCAAAGT78500.035.564462
AAAGTGC69500.035.3684734
TAGCTGG62650.034.959017
TTGGGAG85850.034.1068846
AAGTGCT73050.034.0353135
AGTGCTG74800.033.4275326
ACTCGGG19050.033.0604864
GGAGGCT90750.032.731029
CAAAGTG80250.032.504793
Produced by FastQC (version 0.11.5)