FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2683729
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG8050.052.5970653
CCGAGTA9450.048.3041422
TACTCGG8600.046.4981083
GAGTAGC23100.046.4341744
CTACTCG8350.045.6502382
AGTAGCT38400.045.3299565
CCCAAAG52900.042.7624471
GTAGCTG38300.042.69826
GCTGGGA78400.042.4363679
GCTACTC20100.042.203981
CCCGAGT11350.041.9374921
TAGCTGG40050.041.7703827
CTCGGGA10850.039.4572225
AAGTGCT45200.038.926745
AAAGTGC46100.038.881134
CCAAAGT50100.038.4175832
TCGGGAG12100.038.060096
AGTGCTG48850.037.2284976
ACTCGGG10700.036.932644
GTGCTGG49850.036.2924737
Produced by FastQC (version 0.11.5)