FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2683729
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9400.054.5002942
TACTCGG9950.053.3761753
CGAGTAG9400.050.4993323
GCTACTC21600.050.0792241
CCGAGTA10250.046.7709852
CCCGAGT10800.046.159981
CCCAAAG61050.044.527231
GCTGGGA81950.044.0449649
AAAGTGC49600.043.967164
AGTAGCT37750.042.4550675
GAGTAGC23700.042.4382554
AAGTGCT52050.042.1685075
CCAAAGT56600.041.6026952
GTAGCTG38300.041.4772576
TAGCTGG39500.041.169077
TCGGGAG14100.040.9994546
CAAAGTG57600.040.0637053
ACTCGGG13500.039.3402184
AGTGCTG55050.039.187496
TTGGGAG60100.038.6317676
Produced by FastQC (version 0.11.5)