FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3700028
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACT757.3127885E-637.700591
GCTGGGA67050.031.7498049
CCCAAAG54800.029.582581
CGAGTAG8800.028.33343
CCGAGTA9950.026.9546472
CGGAGGT9950.026.9211377
CGCGATA350.00883766426.85801944-45
TAGCTGG41200.026.6907187
CCCGAGT12500.026.3904111
CGCGCCC4700.026.0289942
GTGCTGG44750.025.3084537
GAGTAGC24350.025.1159654
AAAGTGC47450.024.2904384
GTAGCTG41400.024.0651656
TGCTGGG51800.024.0412868
CCAAAGT55150.023.9741212
AGTAGCT40550.023.78315
CTCGCGT1000.001749831323.5220684
TAGCGCG500.001760144823.49870356-57
AAGTGCT48000.023.228045
Produced by FastQC (version 0.11.5)