FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3283292
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2401.3766621E-6158.23573245
CGAGTAG23300.076.003743
AGTAGCT84700.075.887415
GAGTAGC55400.071.571434
GTAGCTG88350.071.200056
TACTCGG27550.070.555433
CGGTACG750.002193416570.2519220-224
CTACTCG28050.069.9354552
CCGAGTA25400.069.015722
AAGTGCT103350.068.7124945
GCTGGGA200850.067.001969
TAGCTGG98450.066.0163967
TTGGGAG132850.064.001826
GCTACTC61650.063.3716241
GCGCTAT2900.003345639463.143867240-244
AAAGTGC112300.063.022964
CCAAAGT120750.062.909572
CCCAAAG131550.062.027151
CAAAGTG119700.060.970773
ACCCGCA7806.4986543E-660.859898245
Produced by FastQC (version 0.11.5)