FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4038349
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12100.032.628473
CTACTCG9800.032.6126172
CCGAGTA13300.031.09812
GCTGGGA80650.030.419939
CCCGAGT15750.029.8552881
TACTCGG10450.029.6845533
GAGTAGC27400.028.3028954
GCTACTC24800.028.0615621
AGTAGCT43650.027.9953925
CGCCGTA3000.026.63297894
CTCGGGA12200.026.1966955
GGAGGCT59150.026.0622089
TTGGGAG58150.026.0257726
TCGGGAG13050.025.5708586
GTAGCTG44850.025.5696536
TAGCTGG47150.025.5185347
GGGAGGC68600.025.418038
GTGCTGG50950.025.3679837
CCCAAAG64500.025.1513461
AAAGTGC55400.022.736444
Produced by FastQC (version 0.11.5)