FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4107627
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGG1101.7789716E-938.4971775
CGGAGGT9650.030.6801917
CCCGTTA1701.1823431E-1030.4997541
GCTGGGA66750.030.0622469
CCCAAAG58050.028.5820241
GGCGGAG11850.027.7944955
CTCGCGT1205.797825E-627.4470624
GTGCTGG45350.027.1499357
CGTCGAG1406.216196E-726.9348471
CGCGCCC4100.026.4005552
AGGCGGA11950.026.380684
TACTCGG6100.026.2257633
GAGTAGC20550.025.8729344
TTCGCGG1101.01131714E-425.6647854
GCTACTC18950.025.6200851
CCGAGTA8500.024.9151022
TAGCTGG39350.024.721257
AGTAGCT36400.024.3016385
CTACTCG6800.024.2230172
AGTGCTG52100.024.1736326
Produced by FastQC (version 0.11.5)