FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4107627
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8900.041.1914253
CTACTCG9250.038.6166082
CCGAGTA8500.034.8356482
CGGAGGT12900.034.2479937
CGAGTAG8550.033.53253
GCTGGGA69800.033.19589
CCCGAGT10850.032.0739751
GGAGGCT55800.031.4171169
GCTACTC22700.030.6610221
CCCAAAG65450.029.8905871
AGGCGGA19000.029.684514
CTCGGGA12650.028.9801775
GCGGAGG15100.028.946966
GGCGGAG19400.028.8298365
GAGTAGC22250.028.3059064
ACTCGGG12400.028.0486724
GTGCTGG50700.027.254387
TTGGGAG56550.026.9283266
GGGAGGC67050.026.6364578
CGCGCCC4950.026.5861282
Produced by FastQC (version 0.11.5)