FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4217364
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG3450.050.556021
ACGTCGA1658.367351E-1131.4267121
TCGCGTT600.004141355431.3655385
CGCGACT1205.7142915E-627.4983751
CTACTCG7700.026.8927272
TACTCGG7600.026.000383
CGAGACT19700.025.6039981
GTCGAGA7150.024.3539032
GCTGGGA69100.022.3069469
CCGAGTA9600.020.0995122
GACTTGT30950.020.0658364
CGAGTAG9450.019.9146293
CTCGGGA11000.019.6747475
CTCGCGT1456.558389E-419.4682664
GCTACTC21900.019.3726331
CCCAAAG60350.019.0591161
CCCGAGT12750.018.8560281
GTGATCA32700.018.2515729
GTGCTGG49900.018.1761157
GAGTAGC23300.018.1731684
Produced by FastQC (version 0.11.5)