FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4217364
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG10200.032.715643
CTACTCG9700.031.9793362
GCTACTC24100.029.8576031
GCTGGGA77150.025.3425059
CTCGGGA12500.024.8156725
CGAGTAG9300.024.258033
CCCAAAG65900.023.836451
GGAGGCT64200.023.719319
CCGAGTA10050.023.383052
ACTCGGG13550.023.2395444
CGGAGGT12600.023.1266787
GTGCTGG55600.022.4853137
TCGGGAG14300.022.0206876
TTGGGAG52700.021.7606226
GAGTAGC22850.021.3914034
TGACGCG1100.003060906421.3636073
GGGAGGC67600.021.2053418
GGCGGAG15800.021.1199745
AAGTGCT58900.020.986145
CCAAAGT66000.020.7226982
Produced by FastQC (version 0.11.5)