FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4582068
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT601.5933947E-647.0546345
CGTCGAG2050.041.3936041
CGCGAGT1102.9016228E-629.9999081
CGGAGGT8650.028.2504467
GCTGGGA60600.027.2190259
TACTCGG6000.025.8800473
AGGCGGA11450.025.4793644
GGCGGAG11650.025.4458525
CCCGAGT10200.024.9579051
ACCGCGC3050.024.7306021
CCCAAAG60250.024.647231
CGCGCCC4100.024.1069492
CCGCGCC5150.022.8848111
TTCGGCG1452.512751E-522.7160285
GTGCTGG45550.022.6972057
ACGTCGA1252.3757092E-422.6285021
CTACTCG6250.022.5916542
ACTCGCG1252.405974E-422.5862243
CGCGTTG1252.4266899E-422.5576326
GCGGAGG10450.022.4856786
Produced by FastQC (version 0.11.5)