FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4582068
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT13450.038.0897
TCGCGTT906.1959327E-736.5553475
CTACTCG8700.036.195592
TACTCGG8800.035.2501833
GGCGGAG15800.035.1010675
GGCCGGT4400.032.045277
CTCGCGT1501.0713848E-931.3334984
GCTGGGA71800.031.2230329
AGGCGGA17950.030.6352584
GCGGAGG14650.030.1568266
GCTACTC20850.029.9996321
CCCAAAG68300.029.5397591
CGAGTAG8150.029.4111963
CCCGAGT11950.029.122871
GGAGGCG17150.028.775662
ACCGCGC3800.028.4652231
CCGAGTA9000.028.2001462
GTGCTGG54950.028.0544367
GGAGGCT55900.027.8289859
CGTACCG856.819011E-427.6472033
Produced by FastQC (version 0.11.5)