FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3353290
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG8650.044.0655672
CGAGTAG9050.043.147563
TACTCGG8850.041.4646533
CCGAGTA9700.041.2361072
CCCGAGT11000.040.692971
GCTACTC20750.039.9652941
CCCAAAG64200.039.118361
GCTGGGA82000.038.340089
AAAGTGC52700.037.9406744
GAGTAGC21750.036.988234
CCAAAGT61900.035.274252
GTGCTGG55950.034.7729037
AAGTGCT57250.034.5965925
CGCGATA550.00270462234.2155883
CGTACCG550.00270462234.2155883
AGTGCTG58850.033.0598686
TCGGGAG12050.032.7596666
CAAAGTG64100.032.4407543
AGTAGCT38450.032.302365
TAGCTGG40350.031.911547
Produced by FastQC (version 0.11.5)