FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3432766
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG10350.042.3903473
CTACTCG10500.040.8861772
GCTACTC25350.037.0794641
TCGGGAG14200.037.0534256
CCCAAAG68400.036.8069151
GCTGGGA98250.036.0516249
AAAGTGC59050.035.552044
AGTAGCT45500.035.1489755
CCAAAGT66300.034.1548272
GTAGCTG46000.034.0083126
CGAGTAG12000.033.8097233
CCGAGTA12200.033.6421552
AAGTGCT61350.033.604025
GAGTAGC27700.033.2107934
CAAAGTG65600.032.6494873
TAGCTGG50700.032.615257
CTCGGGA14450.032.3215185
AGTGCTG64200.032.050726
GTGCTGG63800.031.8825667
GGAGGCT71550.031.1210869
Produced by FastQC (version 0.11.5)