FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3421746
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8250.041.1564563
CCGAGTA9700.040.3520662
CGAGTAG9400.039.6331563
CTACTCG8650.037.6177142
GAGTAGC23550.037.4464044
AGTAGCT39700.034.6858065
GCTGGGA80450.033.342439
GTAGCTG41700.032.896756
CCCGAGT13400.032.7750361
GCTACTC21350.032.5150571
TAGCTGG44300.032.1311347
CGGAGGT11500.030.637317
ACTCGGG12250.029.6424484
GGAGGCT53850.029.4861589
CTCGGGA12350.029.4024285
TCGGGAG12600.029.08246
CCCAAAG61350.028.4808031
CTCGCGT1005.2057905E-528.2950634
AAAGTGC53600.027.7143824
GGGAGGC68500.027.4319448
Produced by FastQC (version 0.11.5)