FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3421746
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9350.043.1736533
CTACTCG9300.042.396332
CCGAGTA10250.041.672492
CGAGTAG9850.041.4586333
GCTGGGA77700.037.8772549
GCTACTC23100.037.0562631
GAGTAGC23600.036.7952424
CCCGAGT12200.036.2385061
AGTAGCT39350.034.7120445
GTAGCTG40700.033.675996
GGAGGCT62450.033.2968569
CCCAAAG61650.031.7367521
TAGCTGG46700.031.2590267
GTGCTGG51400.031.2316477
TTGGGAG59500.030.8454936
AAAGTGC52350.030.7545494
GGGAGGC73150.030.094738
ACTCGGG12950.030.0843244
TGCTGGG59450.029.845028
AGTGCTG55900.029.6411366
Produced by FastQC (version 0.11.5)