FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3479060
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG8750.035.0148623
CCGAGTA9700.033.043372
TACTCGG7350.032.064893
CCCGAGT12050.030.161031
CTACTCG8250.029.1382452
GCTGGGA70250.027.618929
CCCAAAG57550.025.916971
CGGAGGT10950.024.4547757
TCGGGAG10850.024.2482246
CCAAAGT56300.023.358392
CTCGGGA10900.023.3514795
GGAGGCT50750.023.3274579
GAGTAGC23400.023.164834
GTGCTGG49050.022.7950697
GTAGCTG37450.022.7064066
AGTAGCT36600.022.6661995
GCTACTC19500.022.5107941
AAAGTGC51450.022.4454254
TAGCTGG39400.022.2970967
TTAGGCG1754.3331147E-621.547613
Produced by FastQC (version 0.11.5)