FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3479060
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG8200.033.775653
CCGAGTA8800.031.4727612
TACTCGG8950.030.420793
CTACTCG9250.028.9266832
CTCGCGT1354.6892274E-727.8177344
GCTGGGA67200.027.592259
CCCGAGT11250.026.749621
CCCAAAG52950.026.0190731
GCTACTC22450.025.971411
GAGTAGC21900.025.7218764
TCGGGAG13150.024.6310336
GGAGGCT54800.024.1561589
GTGCTGG45300.023.9371857
AGTAGCT35300.023.8033245
CTCGGGA12450.023.3766145
CGGAGGT11300.023.2631687
GTAGCTG34700.022.99746
GGGAGGC62500.022.9075768
CCAAAGT52700.022.6250042
TTGGGAG53300.021.8415646
Produced by FastQC (version 0.11.5)