FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364258
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8050.040.821783
CTACTCG8350.039.9173432
CGAGTAG8450.038.3338243
CCGAGTA9550.032.935272
GCTACTC20150.032.2012021
GCTGGGA71350.031.0545239
GGAGGCT52700.030.3753019
CCCGAGT12000.030.1701531
ACTCGGG11100.029.6049844
GAGTAGC22400.029.1310754
AGTAGCT36150.028.6990345
GTAGCTG35950.028.3363656
TCGGGAG11850.028.1269936
TTCGGCG856.866228E-427.614325
CTCGGGA11150.027.366665
CCCAAAG56600.026.7489951
GGGAGGC64000.026.3324558
TAGCTGG39750.025.9817777
TTGGGAG53000.025.8636786
AAAGTGC49000.024.6221984
Produced by FastQC (version 0.11.5)