FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3557105
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7400.035.519913
CCGAGTA7750.034.5214232
CTACTCG6900.030.6110972
CCCGAGT10300.030.132061
GCTGGGA62550.029.414549
TACTCGG7200.029.3356363
GCTACTC19600.029.270211
GGAGGCT47650.028.5652759
GAGTAGC19150.028.4318224
TTGGGAG48100.026.6395556
CCCAAAG56000.026.5351241
CGGAGGT9250.025.8784267
AGTAGCT34450.024.9328125
GTGCTGG45000.024.9284237
TGCTGGG47850.024.6203988
GGGAGGC56400.024.3000418
GTAGCTG34500.024.0803936
TAGCTGG35900.024.0565197
AAAGTGC47100.023.7176444
AAGTGCT49950.022.927875
Produced by FastQC (version 0.11.5)