FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4116909
Sequences flagged as poor quality0
Sequence length35-351
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG23300.068.083793
AGTAGCT81700.064.585545
GCGATCG2150.00313911764.174324290-299
CCGAGTA24650.063.5369952
GTAGCTG84500.061.8108336
GAGTAGC56800.060.2361374
GCTGGGA174700.059.7159049
TAGCTGG94050.056.8882687
TTGGGAG124650.054.4880186
CCCGAGT30400.054.433541
AAGTGCT106800.052.6795275
TACTCGG22800.051.5930063
CTACTCG22550.050.674572
GGAGGCT123250.048.9585889
AGTGCTG113500.048.4988176
GTGCTGG114500.048.3088877
GGGAGGC163000.047.302448
GCTACTC54400.047.235611
TCGGGAG26200.047.0869336
AAAGTGC120350.046.2457584
Produced by FastQC (version 0.11.5)