FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4116909
Sequences flagged as poor quality0
Sequence length35-251
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG20350.068.085133
CCGAGTA21900.064.427172
AAGTACG3750.003249108363.612545245
GAGTAGC52150.061.0581444
AGTAGCT82600.059.9393925
GCTGGGA174250.059.5611469
ATACGGT5351.777343E-459.450977245
GTAGCTG85600.057.2447366
CTACTCG23200.056.433822
TACTCGG23950.055.728073
TAGCTGG91900.054.7727557
CCCGAGT26300.054.6861841
TTGGGAG128800.054.377676
TCGGGAG26200.053.124856
AAGTGCT105100.052.9732135
GGAGGCT126500.052.6526689
AGTGCTG108050.050.821086
CTCGGGA26750.049.6566475
GCTACTC54200.049.5493661
AAAGTGC113450.048.1225744
Produced by FastQC (version 0.11.5)