FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3912919
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG7400.026.7658543
CGAGTAG8500.024.966473
CTACTCG7600.024.8204672
CGGAGGT9500.024.229887
GGCGGAG10950.024.1177865
CCCAAAG55400.024.0343531
GCGGAGG10000.023.4893476
GCTGGGA65900.022.9535169
CCGAGTA9500.022.834832
AGGCGGA11350.022.4368294
CGGGTAC2103.8817234E-822.3696659
CCCGAGT12700.022.3069731
CGCGCCC4900.022.1358032
GAGTAGC20150.021.53164
GGAGGCT46600.020.9679629
GCTACTC21800.020.5759661
GTGCTGG43250.020.0939067
CCAAAGT60050.019.7117082
CCCGTAG2201.4259258E-619.3158111
CTCGGGA9600.019.1582985
Produced by FastQC (version 0.11.5)