FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3912919
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG8050.029.1561572
CCGAGTA8900.029.0087382
TACTCGG8350.027.5464553
CGAGTAG9100.027.3395023
CGGAGGT10550.024.4715127
GCTGGGA68250.024.1403719
CCCGAGT12100.022.933921
GAGTAGC23150.022.9130864
GGAGGCT51250.022.805789
GGGAGGC58700.022.6304598
TTGGGAG50700.022.4982596
CCCAAAG58550.022.492761
GTCGCCG1903.4602635E-722.38328294
CTCGGGA10500.021.9057125
GGCGGAG13950.021.8720635
TAGCTGG39400.021.564187
AGTAGCT37100.021.2562165
CCGGGTT6900.021.0890798
CCGATAG1354.05962E-420.9039881
GTAGCTG37650.020.6963466
Produced by FastQC (version 0.11.5)