FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3674380
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7350.038.4958843
CCGAGTA8750.036.6480832
CTACTCG7700.036.133642
GCTGGGA66900.034.829099
TACTCGG7750.034.0750663
CCCGAGT10800.032.7939261
GCTACTC21250.032.4451561
GTGCTGG45150.031.6302367
GGAGGCT50450.031.5664049
CCCAAAG59250.031.4821721
GAGTAGC21100.031.0658174
AGTGCTG50650.028.2902346
AGTAGCT35900.028.2419325
TGCTGGG52100.028.2223518
CGGAGGT10000.028.1862837
GTAGCTG37350.027.9240536
AAAGTGC51150.027.566144
TAGCTGG40300.027.1604797
CGGCGTT700.00869551226.9471192
CCAAAGT59600.026.6645372
Produced by FastQC (version 0.11.5)