FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3674380
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG7900.046.9363373
CTACTCG7600.046.9363332
GCTACTC20750.039.2002071
CGAGTAG8200.037.7780273
CCGAGTA8800.037.335722
CCCGAGT9650.037.02941
GCTGGGA66100.035.5040369
GAGTAGC20000.034.7328874
CGGAGGT11750.033.9539457
CTCGGGA11850.032.8752375
GGAGGCT57450.032.4346859
CCCAAAG59500.032.240631
TCGGGAG12400.031.7955826
ACTCGGG12650.031.1672124
GGCGGAG19350.030.5631945
AGGCGGA18900.030.545874
GGGAGGC70250.030.4000458
GTGCTGG46400.030.1444597
TAGCTGG35800.030.0235217
TTGGGAG57400.029.7645056
Produced by FastQC (version 0.11.5)