FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3265613
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT901.2778401E-841.9138075
CTCGCGT1204.0799932E-935.364784
CCGAGTA9100.033.1625752
CCCGAGT11650.031.60331
CGAGTAG9000.029.863593
ACTCGCG1303.241239E-729.0172523
TACTCGG7100.028.5574723
GAGTAGC22550.028.0199854
GCTGGGA64550.027.7272119
CCCAAAG53500.025.3216421
CGCGTTG1301.0926613E-525.2956286
GTAGCTG35750.025.2299236
CTACTCG7300.025.1913472
TAGCTGG37700.024.796437
GGAGGCT45100.024.7900779
TCGGGAG10000.024.4283476
GTGCTGG39900.023.9001487
AGTAGCT37250.023.6714555
ACCGGTT2002.3139364E-823.4880778
CTCGGGA9250.023.4490765
Produced by FastQC (version 0.11.5)