FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5639606
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG36950.067.7217943
CCGAGTA39550.064.5447542
AGTAGCT130400.064.5285955
GTAGCTG134800.061.2066156
GAGTAGC88150.060.706334
GCTGGGA290350.057.852239
AAGTGCT154750.056.6966175
CCCGAGT46600.055.5251541
TTGGGAG211750.055.484426
TAGCTGG152800.055.274677
CTACTCG38000.054.903092
TACTCGG40450.052.3568083
AGTGCTG168650.052.135856
GGAGGCT214150.051.0358969
AAAGTGC173650.049.9450234
CCAAAGT186150.049.0634162
GCTACTC85600.048.658871
CCCAAAG207750.048.5734861
TTTGGGA193000.048.5626375
GTGCTGG179800.048.516687
Produced by FastQC (version 0.11.5)