FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3696259
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG7000.043.1152763
CGAGTAG10150.040.8851783
CTACTCG7650.039.451892
CCGAGTA10900.039.3698842
CCCGAGT12600.038.2159771
CGGAGGT11300.037.8344767
GCTGGGA75300.034.3156669
GAGTAGC24350.032.729324
CCCAAAG58850.032.3276331
GTAGCTG39600.031.914916
GCTACTC20050.031.7859151
GGAGGCT52250.031.2908749
CTCGGGA9950.031.2802415
AGTAGCT40550.031.0505775
GGCGGAG14750.030.692235
GCGGAGG12700.030.3351866
AGGCGGA15050.030.0804254
TAGCTGG41650.030.0048477
GGGAGGC67350.029.8559538
TCGCGTT804.6902322E-429.4733335
Produced by FastQC (version 0.11.5)