FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3696259
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG7850.044.847063
CTACTCG8150.042.62032
CGAGTAG8600.041.4817963
CCGAGTA9100.039.2025762
CGGAGGT12150.036.3156627
GCTGGGA73650.035.881639
CCCGAGT11650.035.5201951
GCTACTC23900.033.6447071
CCCAAAG61850.032.9205251
GAGTAGC24150.031.8763874
AGTAGCT41100.031.8643285
TCGGGAG11750.031.5596086
GGAGGCT55800.031.46119
GGCGGAG18700.030.8749225
GTAGCTG39900.030.7050676
TTGGGAG59050.030.5248626
TAGCTGG42650.030.0459567
AGGCGGA19700.030.0224914
GTGCTGG50750.029.4126017
GCGGAGG15650.029.393696
Produced by FastQC (version 0.11.5)