FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2195128
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG7950.045.063823
CTACTCG7450.044.9245382
CCGAGTA8150.042.222772
CGAGTAG7650.040.669053
GCTACTC19850.040.1868361
GCTGGGA72650.039.1214459
AAAGTGC41250.038.9683464
CCCAAAG49350.038.7369541
CCAAAGT45300.038.5021552
GAGTAGC19450.037.8082474
AAGTGCT43150.037.361725
AGTAGCT31750.036.820485
CAAAGTG46050.035.5206873
CTCGGGA10650.035.409675
TAGCTGG37400.035.1707427
GTAGCTG33750.034.9385576
AGTGCTG45300.034.8454486
ACTCGGG10450.034.7340934
TCGGGAG11150.033.7070056
GGAGGCT54300.033.4816069
Produced by FastQC (version 0.11.5)