FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2195128
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9250.047.1962972
TACTCGG9250.047.1962973
CCGAGTA9350.046.6915252
GCTACTC20650.045.775681
CGAGTAG9400.044.9449963
AAAGTGC40050.042.547184
CCCAAAG48950.042.0804371
GAGTAGC20700.041.953494
AAGTGCT41750.040.8137975
GCTGGGA73650.040.6634989
CCAAAGT47750.040.3066942
AGTAGCT33600.038.9781955
GTAGCTG33800.038.7475556
CAAAGTG47550.038.5017783
AGTGCTG44000.038.4066666
TAGCTGG37050.037.75597
CCCGAGT11850.037.7019771
TTGGGAG53150.037.270576
GTGCTGG44950.037.0728037
GGAGGCT60850.036.3343289
Produced by FastQC (version 0.11.5)