FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3498500
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA25650.072.1585852
CGAGTAG25200.070.2653053
AGTAGCT89200.068.764965
GTAGCTG91600.065.796146
GAGTAGC58500.064.8765954
GCTGGGA188800.061.8980879
TTGGGAG141200.061.6120536
CCCGAGT31250.059.729671
AAGTGCT98000.059.4538235
CTACTCG29150.059.36862
TAGCTGG104850.058.9471327
TACTCGG29250.058.4803123
TCGGGAG32250.057.80486
GCACTTT118550.055.586661
CTCGGGA31800.055.050795
TTTGGGA130000.054.9444435
GGAGGCT149100.053.955819
ACTTTGG119550.053.8794363
AGTGCTG107300.053.7403456
CACTTTG122400.052.9524352
Produced by FastQC (version 0.11.5)