FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2951749
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10400.046.224843
CCGAGTA11250.043.151242
GCTGGGA84600.041.760289
CCCGAGT13200.040.028211
GCTACTC21700.040.0018581
GAGTAGC24400.039.5979424
CTACTCG9550.039.481642
TACTCGG9700.039.356993
GTAGCTG42600.037.9384426
AAAGTGC48500.037.8993234
AGTAGCT42150.037.6826025
CCCAAAG58600.037.676481
AAGTGCT49850.037.1565975
TAGCTGG47900.035.5049557
GGAGGCT62250.035.16929
AGTGCTG52150.034.684636
TCGCGAA550.002719892434.17618694
GTGCTGG54450.034.16757
CCAAAGT58100.033.827392
TTGGGAG56900.033.523126
Produced by FastQC (version 0.11.5)