FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122609
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG8300.051.7115253
CCGAGTA8950.050.590882
TACTCGG7550.046.2283943
CTACTCG7750.045.643992
CCCGAGT11100.044.6761971
GCTGGGA75800.042.577419
GAGTAGC21050.042.348084
CCCAAAG50250.042.1067661
AAAGTGC42900.041.6683164
AAGTGCT44150.041.236395
GCTACTC17450.040.868761
AGTAGCT34950.040.350425
AGTGCTG45250.040.076626
GTAGCTG35450.039.6257366
CCAAAGT50250.039.60962
TAGCTGG37500.039.461267
CAAAGTG48500.038.9965933
TTGGGAG49000.037.105416
GTGCTGG50500.035.6286247
TCGGGAG12100.035.332836
Produced by FastQC (version 0.11.5)