FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4871478
Sequences flagged as poor quality0
Sequence length35-251
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT104900.059.3220065
CGAGTAG29550.058.773823
GAGTAGC68000.057.678584
CCGAGTA30800.057.636972
GTAGCTG106750.057.273356
GCTGGGA215300.057.27029
CTACTCG31100.054.4020922
TACTCGG31650.053.254233
TTGGGAG169050.053.1122936
CCCGAGT35450.053.032891
GGAGGCT163650.051.1048479
TAGCTGG124850.050.150837
AAGTGCT127950.049.536815
TCGGGAG35800.048.8710566
GCTACTC72300.048.0123441
CTCGGGA34200.047.9717945
AGTGCTG131850.047.7799266
AAAGTGC137300.046.0700534
GTGCTGG135650.045.780037
GGGAGGC230800.045.399818
Produced by FastQC (version 0.11.5)