FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2887876
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1500.043.9245951
CCGCTTA950.001300308424.7523382
CCGAGTA6600.022.0895882
GCGAACG1200.005027442219.6091941
CGAGTAG6500.019.5207633
GCTGGGA58800.018.2223329
AGTAGCT27150.018.1746435
CCCGAGT9100.017.583631
GAGTAGC16450.017.4265084
CCCAAAG45200.017.3879761
TAGCTGG30700.016.8383967
TATTACG1700.001921104516.5862713
GTAGCTG29800.016.5584416
GGAGGCT42150.016.3895199
GCTACTC15100.016.2068041
AAAGTGC34550.016.1862164
CCTATAG9200.015.857871
CCGGGTT6000.015.6648128
AAGTGCT37300.015.6228145
TTGGGAG35500.015.35592656
Produced by FastQC (version 0.11.5)