FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2887876
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG500.001700964337.60675
CCGAGTA7200.028.0749322
CGAGTAG7500.025.0711333
TACTCGG5800.023.5041873
CTACTCG5700.023.0922342
CCCGAGT10700.021.967671
GAGTAGC16850.021.4815714
AGTAGCT28300.020.597315
GCTGGGA57450.020.1271699
TCGCCGT3553.6379788E-1219.8606394
GCTACTC16100.019.2715151
TAGCTGG32450.019.2645787
GGTCGCC4900.019.18509794
ACTCGGG8400.019.0271994
GTAGCTG30750.018.8023726
GTCGCCG3300.018.51652594
CCGCTAT2051.739587E-518.3450492
CTCGGGA8200.018.344735
CCCAAAG42050.018.1111831
AAAGTGC35500.017.0819174
Produced by FastQC (version 0.11.5)