FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605777
Sequences flagged as poor quality0
Sequence length35-251
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGTG2954.7519934E-4103.207855245
AGTAGCT60200.072.036655
GTAGCTG60900.070.736956
CCGAAAA5755.16593E-970.60016245
CGAGTAG16650.070.190513
CCGAGTA17500.068.418052
TAGCTGG67400.065.105057
TACTCGG18700.064.64033
CTACTCG18200.064.527522
GAGTAGC38300.064.317944
AAGTGCT71400.063.7054335
GCTGGGA137750.062.795889
GCTACTC43100.061.809451
TTGGGAG93250.060.3879786
ACGCTCG1709.943829E-459.69866245
AGTGCTG77000.058.922386
AAAGTGC76800.058.257564
ACAGCGA7002.0057839E-457.992985245
CCAAAGT83300.056.943982
GGAGGCT107700.055.8495189
Produced by FastQC (version 0.11.5)