FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3269359
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGT2350.0045310175168.34656320-329
CCGGTTT6750.00796252858.609543320-329
AGTAGCT53900.055.4143335
CAACGTA4250.001569855.066853340-345
GTCGCGT1102.9540545E-453.65367180-189
CGAGTAG15100.051.6339263
GTAGCTG59300.050.9482276
GCTGGGA117200.049.9915359
TAGCTGG62850.049.4387177
CCGAGTA15900.049.4116672
GAGTAGC36700.049.2059564
TACTCGG12800.045.683923
AAGTGCT66400.045.6731265
CTACTCG12000.044.921342
CCGGTAT3450.00410007644.74902290-299
TTGGGAG75850.042.7037546
CCCAAAG90150.042.581961
GCTACTC30250.041.9210471
AGTGCTG71000.041.8258326
TCGGGAG17150.041.7848746
Produced by FastQC (version 0.11.5)