FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3269359
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGA1500.0046842047166.51599245
CGGTACG1301.68669E-460.095406220-224
CTACTCG14500.057.881252
ATCGACG2050.005119748456.715706220-224
TACTCGG14950.055.7663653
TACGGCA4400.00591759154.66828225-229
CGAGTAG13450.054.134153
AGTAGCT51400.053.8503465
GAGTAGC32700.051.841124
TTGGGAG89000.051.2089846
GTAGCTG53200.050.9835976
GCTGGGA122100.049.8881349
AAGTGCT71150.049.7607425
GCTACTC33800.049.3340531
CCGAGTA14750.048.610112
TAGCTGG57550.048.479057
CGTCGAA1305.1581545E-447.92226160-164
GGAGGCT94750.046.632679
AAAGTGC75100.046.6254354
CCAAAGT80350.045.7932052
Produced by FastQC (version 0.11.5)