FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319220
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG4350.001671104775.21003245
TCGTCGA1100.001909085472.75164190-194
CGGTACG950.002161898970.50725220-224
CGAGTAG15800.065.840793
TACTCGG15250.063.3694763
CTACTCG15900.061.8514632
AGTAGCT60750.061.7587745
CCGAGTA17150.061.320912
GTAGCTG62500.059.753066
GAGTAGC41700.059.4362564
GCTGGGA139950.057.553519
AAGTGCT79250.056.953745
GTATTCG3400.005126192756.692364215-219
TAGCTGG69700.055.2932057
GCTACTC38950.055.0226441
AAAGTGC80350.054.0515944
AACGTCG2050.00550361753.197334245
CTATCGG2058.437708E-553.197334245
AGTGCTG84550.052.9770136
CCCGAGT19900.052.562051
Produced by FastQC (version 0.11.5)