FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649146
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG352.9401245E-453.707214
CGAGTAG7850.046.0958373
CCGAGTA8350.042.7954252
CTACTCG6800.042.1785052
AGTAGCT32100.041.5770765
GAGTAGC20750.038.9539034
GTAGCTG32350.038.640976
TAGCTGG35400.038.3657
TACTCGG7600.037.7187163
GCTACTC16800.036.6896861
CCCGAGT10150.036.622081
GCTGGGA68100.035.8146679
TTGGGAG48950.034.7533386
CCCAAAG46350.034.616781
AAAGTGC37600.032.3707354
AAGTGCT39600.031.3292085
GGGAGGC61100.030.9190068
AGTGCTG40350.030.746886
CCAAAGT42850.030.7239782
GGAGGCT51700.030.7232259
Produced by FastQC (version 0.11.5)