FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2850007
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7450.034.6931233
CCGAGTA7350.033.9072272
CCCGAGT8700.031.9194951
TTCGACG600.004152360431.348192
CGTCGCG753.2794426E-431.3289439
TACTCGG5400.030.458693
AGCGTCG804.7865656E-429.3708827
GCTGGGA52800.029.1928779
GAGTAGC16450.029.1387734
GCTACTC14400.029.0904731
AGTAGCT28150.029.0474385
TCGACGG650.006159890428.9190243
CTACTCG5700.028.8733332
GTAGCTG27600.027.9236226
TAGCTGG29700.027.5314967
CCCAAAG41150.026.4219821
TTGGGAG36850.024.4850356
GGAGGCT41100.024.1255729
TCGGGAG7600.024.115046
GGGAGGC46200.023.5984238
Produced by FastQC (version 0.11.5)