FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2850007
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG6350.039.2401122
TACTCGG7150.036.1627853
CGGAGGT9900.034.184277
GCTGGGA52850.033.707249
TTCGGCG702.1826389E-433.5797275
CGAGTAG6850.032.256153
TTGGGAG43550.030.8705926
GGAGGCT43600.030.724689
GTCGCCG2051.8189894E-1229.80701494
CCGAGTA7450.029.6598992
GGGAGGC51450.029.3257168
CCCAAAG46450.029.2514781
CCCGAGT9350.029.1643351
GTGCTGG36200.027.7865227
CTCGGGA9150.027.744565
GAGTAGC17350.027.6379574
GGAGGTT28000.027.5305448
TAGCTGG29600.027.4715637
GGCGGAG13050.027.018175
GCGGAGG12200.026.9715126
Produced by FastQC (version 0.11.5)