FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3467301
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT76100.068.4085545
CGAGTAG22100.064.243823
CCGAGTA22300.063.9611852
GTAGCTG80550.063.2162446
TAGGCGG5201.1526936E-862.90565280-289
AAGTGCT81650.061.1906665
GAGTAGC52650.059.5084464
TAGCTGG89750.059.205837
GCTGGGA168850.059.072929
TAAGCGG3103.8848357E-653.883923340-345
CTACTCG19050.053.4808582
TACTCGG20000.053.316953
CCAAAGT103300.053.2582362
AGTGCTG93450.053.1435476
AAAGTGC93600.052.9943774
CCCAAAG116900.052.431731
CGCGTGG5300.00675982851.567642310-319
CCGCCTA4600.00764351151.244923330-339
CAAAGTG102850.050.7328383
CAAGTAG37700.050.213563
Produced by FastQC (version 0.11.5)