FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3467301
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG2352.1235215E-591.28759200-204
CGAGTAG20500.066.512493
AGTAGCT74850.066.025255
GAGTAGC49250.063.4311144
GTAGCTG79200.061.963026
CCGAGTA22850.061.686282
TACTCGG20100.060.966643
AAGTGCT87200.060.8307465
GCTGGGA171200.059.2449049
TAGCTGG86350.059.163397
CTACTCG21450.058.738862
GCTACTC50500.058.10321
AAAGTGC91450.056.8713384
TTGGGAG117800.054.992296
AGTGCTG95950.054.8636676
CAAAGTG99750.054.0424963
CCAAAGT102550.053.9133642
GGAGGCT136950.052.9731149
CCCAAAG112350.052.8479161
ACATCGA5950.007289651351.857773245
Produced by FastQC (version 0.11.5)